Gene structure prediction is from Flybase release 3.1 (The FlyBase Consortium, 2003) and from PCR 5′ and 3′ RACE analyses and sequencing. Predicted structure of the iav protein and the iav mutant alleles. C, CG4536 gene structure and lesions in the iav alleles.

The left extent of the candidate region is defined by the lethal P insertion l(1)G0254 in cytogenetic region 6C5 and the right by the gene ogre in 6E4, because Df (1)3196 ( Df(1)Sxl-bt) removes ogre yet complements iav (Curtin et al., 1999). The circles show P-element insertions, and inverted triangles a-d represent sequence polymorphisms used for recombination mapping (see Materials and Methods). Predicted genes are shown above and below the line representing the genetic region. B, The iav candidate region at higher resolution assembled from Flybase (The FlyBase Consortium, 2003). This insertion is not rescued by Dp(1 3)sn, indicating that it is left relative to the duplication. The left edge is defined by the lethal P element insertion l(1)G0254 (Schaefer et al., 1999), which is in the predicted gene CG4094 in 6C10-11 (data not shown). The right edge of the candidate region is defined by the gene ogre, which complements iav and is to the left of Df(1)3196 but is uncovered by Df(1)947 (Curtin et al., 1999). The indicated deficiencies and duplication were crossed to iav 1 and scored for ability to complement the locomotor inactivity phenotype. A functional green fluorescence protein-IAV fusion protein shows that the channel is restricted to the proximal cilium, constraining models for channel activation. IAV and NAN are each undetectable in cilia of mutants lacking the other protein, indicating that they both contribute to a heteromultimeric transduction channel in vivo. The IAV protein forms a hypotonically activated channel when expressed in cultured cells in flies, it is specifically expressed in the chordotonal neurons, localized to their cilia and required for hearing. Here, we show that the only other Drosophila TRPV protein is mutated in the behavioral mutant inactive (iav). which are similar to the chordotonal neurons (CNs) in the Johnston’sorgan(JO. (Iav) and nanchung (Nan) which form a functional hetero. Bipolarneurons contain TRP channels sensitive to odors, chemicals, light, stretch, and sound. We previously found that a Drosophila protein in the vanilloid receptor subfamily (TRPV) channel subunit, Nanchung (NAN), is localized to the chordotonal cilia and required to generate sound-evoked potentials (Kim et al., 2003). Bipolar neurons have one axon and one dendrite separated by the soma (Orgogozo and Grueber, 2005). Hearing in Drosophila depends on the transduction of antennal vibration into receptor potentials by ciliated sensory neurons in Johnston's organ, the antennal chordotonal organ.

Chordotonal neuron dendrite iav